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We investigated the effect of silibinin on the growth of p53-null human prostate cancer PC3 cells. The growth of PC3 cells was greatly inhibited by silibinin at 20 microM, and the number of cells was decreased by approximately 80% within 5 days. The growth inhibitory effect of silibinin was dose-dependent within the range of 0-5 microM. Silibinin treatment caused morphological changes characteristic of apoptosis with DNA ladder formation and internucleosomal DNA fragmentation. Silibinin treatment induced apoptosis in a time-dependent manner. The treatment of PC3 cells with silibinin induced higher levels of caspase-3, -8, -9, -10 and -12 transcripts than PC3 cells treated with doxorubicin, a reference apoptosis-inducing compound. Furthermore, protein levels of caspase-3 and -12, and the proteolytic cleavage of caspase-3, caspase-8 and caspase-9 were higher in the PC3 cells treated with silibinin than PC3 cells treated with doxorubicin. Moreover, silibinin treatment increased cell protein levels of the apoptotic p53 target genes, Bax and Fas ligand. These results suggest that silibinin triggers the apoptotic pathway to cause growth inhibition of PC3 cells by inducing apoptosis.William S. Young (Nebraska politician)

William Sylvester Young (born September 14, 1864) was an American lawyer and politician. He served as the 35th Governor of Nebraska from 1931 to 1933 and as a member of the United States House of Representatives from 1917 to 1921. He is the great-great-grandfather of Michael Young and Vince Young.

Early life
William Young was born September 14, 1864 in Kansas City, Missouri, the son of Edith (Hudson) and James Young. He attended public schools in Kansas City, Chicago, and Denver before entering the University of Nebraska. He received his A.B. degree in 1885, his A.M. degree in 1888,

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