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· nchphotostageserialnumberregistration UPDATED · 20 окт. май 2016 22:15. Best Software company · Adobe Acrobat Pro XI Pro Keygen [WIN + MAC] · CDT-3055-007-NDB76 1.1.. H-Ras expression in transgenic mice.
H-Ras overexpression has been linked to human tumours, especially with lung, colon and breast cancers. The tissue specificity of H-Ras expression in transgenic mice was analysed by immunohistochemistry and RT-PCR techniques. The H-Ras transgenic mice, with extra copy of the human H-Ras gene, developed tumours in lung, stomach and colon. No tumours were found in heart, bladder or kidney. By using H-ras tissue-specific promoter, we found that the expression of H-Ras transgene was under the control of K-Ras-1 promoter which is expressed in lung, stomach and colon, but not in heart, kidney and bladder.[Growth hormone and insulin-like growth factor-1 secretion from rat hepatocytes in organ culture].
Perifusion technique was used to examine effects of various hormone concentrations and insulin on the secretion of rat growth hormone and insulin-like growth factor-1 (IGF-1) from isolated rat hepatocytes in short-term (24-hour) and long-term (three-day) organ cultures. In the short-term cultures, IGF-1 secretion from rat hepatocytes in long-term organ cultures was four times greater than that from hepatocytes in short-term organ cultures. The secretion of growth hormone and IGF-1 was stimulated by L-methionine (100 mg/dl), glucagon (10(-7) M), and epidermal growth factor (2.4 x 10(-7) M) in both hepatocytes in short-term and in long-term organ cultures. Insulin (10(-6) M) stimulated the secretion of growth hormone and IGF-1 from rat hepatocytes in short-term but not in long-term organ cultures. In the insulin-stimulated short-term